How Does Gas Chromatography Work

Gas chromatography is an analytical separation technique used in the separation and analysis of samples. The separation occurs between a gas mobile phase and a liquid stationary phase. The sample used in the gas chromatography should be able to vaporize without thermal decomposition. The sample of concern is mixed with the mobile phase and injected into the gas chromatograph. After vaporization by heating, the sample enters into the column with a liquid stationary phase. At the end of the column, detectors produce a chromatogram by identifying the compounds progressing down the column.

Key Areas Covered

1. What is Gas Chromatography
      – Definition, Principle, Applications
2. How Does Gas Chromatography Work
     – Process of Gas Chromatography

Key Terms: Boiling Point, Detector, Gas Chromatography, Mobile Phase, Stationary Phase

How Does Gas Chromatography Work - Infograph

What is Gas Chromatography

Gas chromatography is a technique used in the separation of a mixture of volatile compounds based on their mobility through a stationary phase. It uses a gas mobile phase and a liquid stationary phase. The mobile phase can be inert gases such as argon, helium or hydrogen. A thin layer of liquid stationary phase coats the inner side of the column used in gas chromatography. Gas chromatography is mainly used for both qualitative and the quantitative analysis of molecules within a mixture.

How Does Gas Chromatography Work

The sample mixture should be able to vaporize in gas chromatography to move along with the gaseous mobile phase. The molecules of the mixture interact with the stationary phase inside the column. The molecules with fewer interactions with the stationary phase move faster through it while the molecules with higher interaction with the stationary phase move slower through it. Generally, the mobile phase is inert and is non-polar. The compounds having low boiling points and low molecular weights interact more with the gaseous mobile phase. The compounds that have high boiling points and high molecular weights interact more with the liquid stationary phase. Instrumentation of gas chromatography is shown in figure 1.

How Does Gas Chromatography Work

Figure 1: Gas Chromatography

The polarity and the temperature of the column are the other factors responsible for the relative mobility of molecules through the column. If the polarity of the compounds in the mixture is high, they tend to remain in the stationary phase. Hence, nonpolar compounds move out of the column first. If the temperature of the column is high, the vaporization of the compounds in the mixture occurs faster; therefore, they come out of the column quickly.

Gas chromatograph uses several types of detectors such as mass spectrometry, flame ionization detector, thermal conductivity detector, electron capture detector, etc. The detector at the end of the column identifies the molecules that come out of the column and produces a chromatogram with respect to the time taken for the elution, the process of removing an adsorbed material (adsorbate) from an adsorbent with a liquid.

When a particular type of component of the mixture comes out from the column, it is shown as a peak in the chromatogram. The time taken for the elution of a particular component is used to identify the component under a defined set of conditions.

The size of the peak is directly proportional to the amount of that particular compound present in the sample. The first peak is due to the inner carrier gas, which comes out of the column first. The solvent used in the preparation of the sample elutes secondly.


Gas chromatography is an analytical technique used in the separation of a mixture of volatile compounds. It uses a gaseous mobile phase and a liquid stationary phase. The simpler and more inert compounds come out of the column quickly while heavier and polar compounds take some time for the elution.


1. “Gas Chromatography.” Chemistry LibreTexts, Libretexts, 21 July 2016, .

Image Courtesy:

1. “Gas chromatograph-vector” By Offnfopt – Own work created using File:Gas chromatograph.png as a reference. (Public Domain) via

About the Author: Lakna

Lakna, a graduate in Molecular Biology & Biochemistry, is a Molecular Biologist and has a broad and keen interest in the discovery of nature related things

Leave a Comment

Related pages

what is the difference between phenyl and benzylwhat is the difference between intermolecular and intramolecular forcesexplain the difference between genotype and phenotypethe difference between pinocytosis and receptor-mediated endocytosis is thatwhy is uluru famouscompressive and tensile stresswhat is the difference between cookies and biscuitslocation of dna in prokaryotes and eukaryoteswhat does donde eres meanpolar and nonpolar differencesmicroevolution macroevolutiondifferences between formative and summative assessmentworking principle of hygrometerinterstate definition intrastatedifference between reactance and resistanceassimilation psychology examplesinterrogative pronouns examples in sentencesjuxtaposition definition and examplesdef of assertivedifference between alpha helix and beta sheetdifference between alpha and beta amino acidsbemused meaningspelling forgoprocess of inhalation and exhalationdifference between caribou and reindeercougar and puma differencewhat subatomic particles are involved in nuclear reactionslion seal factsdrink drank drunk grammarhypomanic vs manicanimal personification exampleswhats an expository essaydifference between formal and informal writingwhat is the difference between sugar and raw sugarallusion in literary termsthe difference between a hurricane and a cyclonedefinition heterotrophsalum singularanthropology and sociology definitionalkynes chemical propertieslayed laidpixies fairiesthe difference between longitudinal and transverse wavesdifference between mitosis and cell divisionpsychosis neurosiswhat is the difference between fair and faredifference between dominant and recessive traitsmeaning of transitive and intransitive verbsis it dying or dieingdefinition of co enzymedifference between anaerobic respiration and fermentationamerican bulldogs vs pitbullsinformal imperialism definitionexplain valencysimilarities of transverse and longitudinal wavesis deoxyribose a sugarundampeddifference between monocot and dicot rootsdefine heterogeneous mixtureseffects of hair rebondinguse restraint in a sentenceexamples of novellasflagella or ciliathe difference between insulators and conductorsdifference between ultrasonic and ultrasoundsn1 sn2 mechanismsnaturalism definition in literaturedifference between artefact and artifactice cream vs custardidiopathic hypersomnolencetill prepositiondefine depersonalizedformation of sigma and pi bondssugarcane and sugar beetsimilarities of mitochondria and chloroplastshow to prepare bank reconciliation statement with example