How to Find Amino Acid Sequence

What is Amino Acid

Amino acids are the basic building blocks of all the proteins in our body. May it be a hormone, an enzyme, a structural protein like keratin, all of these are made up of amino acids. Amino acids polymerize to produce proteins. Since every amino acid has a basic –NH2 end and an acidic –COOH end, these terminals react with each other making a chain of amino acids which is called a polypeptide. A polypeptide may contain a variety of amino acids. Depending on the order of amino acids also known as the amino acid sequence, proteins may differ from each other. The sequencing is of utmost importance because it determines whether the protein functions properly or not.

Amino acids do not polymerize randomly. This process is highly regulated. The code of each protein is stored in a DNA sequence which is first transcripted to a m-RNA sequence (in higher organisms DNA is spliced before converting to m-RNA where the unwanted DNA sequences lying in between genes are removed ) and then m- RNA is translated into an amino acid sequence. 

How to find amino acid sequence | clickspay.ru(A = Adenine, T= Thymine, G= Guanine, C= Cytosine, U= Uracil in m-RNA t is replaced by U)

If we consider DNA as a four letter alphabet and it can make three letter words, these three letter words are called Codons. Each of these codons stands for a particular amino acid. Therefore, if a DNA sequence or m-RNA sequence is provided we may be able to predict the amino acid sequence. The real issue is since DNA is a linear array of information we should have some rules to start and stop at the right position.

Few steps to find amino acid sequence

STEP 1  – Know which DNA strand is given. There are two strands: Coding strand or non-coding strand.

 Amino Acid Sequence 01 | clickspay.ru

 

 

One can either read the coding strand from 3’ to 5’ or read the template strand from 5’ to 3’ when making the corresponding m-RNA strand.

STEP 2 – Write the corresponding m-RNA strand.

Using Coding strand: (A= U, T= A, G=C, C=G) Read from left to right

Amino Acid Sequence 02 | clickspay.ru

 

 

Using template strand: (T=U)Read from left to right

Amino Acid Sequence 03 | clickspay.ru

 

 

We can see that we achieve the same sequence irrespective of the strand used.

STEP 3 – Convert m-RNA as a sequence of codons. ALWAYS start from the codon AUG and NEVER count the same nucleotide twice!

Amino Acid Sequence 04 | clickspay.ru

 

 

 

 

 

STEP 4 – Use the below table to find the relevant amino acid sequence.

Also remember,
a. Start codon AUG stands for Methionine.
b. If you come across a stop codon UAA, UGA, UAG you should stop sequencing.

Check if you end up with the answer shown below:

Met- Leu- Asp- Val- Phe-STOP 

Amino Acid Sequence 05 | clickspay.ru

About the Author: Koshal

Leave a Reply


Related pages


marginal and absorption costing worked examplescompliment vs supplementwhat is the difference between immoral and unethicaldifference between vermicelli and spaghettihepatitis abcde differencedrank in a sentencesoughting meaningsoda bicarbonate vs baking sodadifference between a shark and a dolphinfunction of granasuspension in chemistry definitiondifference between symbolism and imageryis it dieing or dyingstructure isopropyl alcoholflavour definitiondeism vs theismdefine coefficient of kinetic frictionwhat is soldering and brazingdifference between flying ant and termitehypoglycemic and hyperglycemiceuchromatin and heterochromatindifferentiate between bit and bytewhat is the difference between realism and naturalismdefine nucleoplasmeffects of hair rebondingwhat is the difference between ham and bacondystonia vs dyskinesiawarm cold bloodedquenched meaning in hindiwhat's the difference between prokaryotic and eukaryotic cellswhy is uluru importantdifference between metaphor and similealumna vs alumniwhat are the differences between aerobic and anaerobicwhats a narrative essaytensile strength diagramjure definitiondolphins versus sharkshow to write an iambic pentameter poemcold blooded animals wikidefine structuralism in psychologyinsulators and conductors definitiontypes of adverb with examplesbelgian malinois intelligencehow to tell wheat from barleybrandy vs rumpositive vs normative economicscentrioles microtubulesvascular plant characteristicsgerman shepherd belgian malinoisexamples of types of nounslittle red riding hood moralwhat is a volatile substanceacculturation definednucleotide and nucleosidecatholic dogma vs doctrineresonance structures for co32difference between oats and barley in hindiare homologous chromosomes identicalmaid of honor meaningrationalism and empiricism differencesexamples of linking and action verbsdefine consonant soundforgo meaningdistinguish between diverging and converging lenses2d echo testrmr mifflin st jeor equationsecondary succession meaningenthalpy defbronchitis and asthma differencedifference between baking soda and bicarbonate sodaaliphatic hydrocarbon definitionpassive lpfthe difference between absorption and adsorptionsynesthesia examples in poetry